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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Ann Biomed Eng. 2021 Feb 9;49(9):2114–2125. doi: 10.1007/s10439-021-02736-9

Figure 4. Cellular bioactivity assay of ALP upregulation.

Figure 4.

A) ALP activity normalized by DNA content for W-20-17 cells. Horizontal dotted line indicates averaged value for control cells incubated in culture medium without added growth factor; grey box represents ± standard deviation of these controls cells’ values. * denote significant upregulation (p < 0.05) from the averaged value for control cells as determined by unpaired t-tests. B) Calibration curve derived from four-point logistic regression which correlates normalized ALP activity of cells incubated with known concentrations of bioactive BMP-2 in ng/mL. C) Cumulative release profile of bioactive BMP-2 calculated from normalized ALP measurements for 3D printed constructs and PLGA delivery vehicles. Data are reported as mean ± standard deviation for n = 3-4 samples. Legends refer to printing temperature or non-printed PLGA particle control.