Table 1.
Primers for the 15 candidate genes and reference genes used for the quantitative PCR (qPCR) analysis in the breast muscle of normal and White Striping (WS)-affected broilers.
| Gene | Ensembl ID | Primer (5'–3') | Size (bp) |
|---|---|---|---|
|
ACTG1 Actin, gamma 1 |
ENSGALG00000028749 | F: 5'-CTCTGTTCCAACCCTCTTTCCT-3' R: 3'-GTGTTGGCGTACAGATCCTTC-5' |
112 |
| CA2 | ENSGALG00000030781 | F: 5'-GCTCTGAGCAGATGTGCAAAC-3' R: 3'-CTCATCGCTGAGGTTACTGGAAG-5' |
144 |
| Carbonic anhydrase 2 | |||
|
CALM2* Calmodulin 2 |
ENSGALG00000010023 | F: 5´-CCACCATGGCTGATCAACTG-3' R: 5´-GCCATTGCCATCAGCGTCTA-3´ |
191 |
|
PLIN1 Perilipin 1 |
ENSGALG00000023395 | F: 5'-GGCTATGGAGACGGTGGATG-3' R: 5'-CTGGCTTGCTCTCCTCTTCC-3' |
173 |
|
CHST1 Carbohydrate sulfotransferase 1 |
ENSGALG00000008440 | F: 5'-CGCCCCTCTTTCTCGTCTTC-3' R: 5'-CTCATCGCTGAGGTTACTGGAAG-3' |
133 |
|
CSRP3 Cysteine and glycine rich protein 3 |
ENSGALG00000004044 | F: 5'-GCTCTGAGCAGATGTGCAAAC-3' R: 3'-GCTTGGAGAGACCCGATTCC-5' |
202 |
|
CTGF Connective tissue growth factor |
ENSGALG00000037402 | F: 5'-TCACCAACGATAATGCTTTCTG-3' R: 3'-GAATGCACTTTTTGCCTTTCTT-5' |
111 |
|
DNASE1L3 Deoxyribonuclease I-like 3 |
ENSGALG00000005688 | F: 5'-GAGTTTGCGTGGCTCATCG-3' R: 3'-CACGATCCTGTCATAGGGGC-5' |
78 |
| HDAC1 | ENSGALG00000003297 | F: 5'-GGGGCGGGTTGCGTT-3' | 115 |
| Histone deacetylase 1 | R: 3'-ACATCACCGTCGTAGTAGTAGC-5' | ||
|
HIF1A Hypoxia inducible factor 1 alpha subunit |
ENSGALG00000011870 | F: 5'-CGTCACCGACAAGAAGAGGATT-3' R: 5'-GTCAGCCTCATAATGGATGCCT-3' |
171 |
| MAPK13 | ENSGALG00000030966 | F: 5'-TCTGCTCCGCCATAGACAAG-3' R: 5'-CAAGCAGCCCAATGACATTCTC-3' |
150 |
| Mitogen-activated protein kinase 13 | |||
| MYLK2 | ENSGALG00000006273 | F: 5'-ACCCTTTTGAGATATTGGACGA-3' R: 5'-TCCTTGGAGCTGAGGTTGTACT-3' |
112 |
| Myosin light chain kinase 2 | |||
| RYR2 | ENSGALG00000010812 | F: 5'-ATACAAGGGACCTGCTGGGT-3' R: 5'-GGGAGGCAAAACAATCTGGC-3' |
92 |
| Ryanodine receptor 2 | |||
|
SMAD3 SMAD family member 3 |
ENSGALG00000035701 | F: 5'-CCCCATGTCATCTACTGCCG-3' R: 5'-GGTAACACTGGGGTCTCCAC-3' |
158 |
| TNNC2 | ENSGALG00000006835 | F: 5'-GTCAATGACGGACCAGCAG-3' R: 5'-CGTCCGCATCAAACATGTCA-3' |
95 |
| Troponin C2, fast skeletal type | |||
|
RPL5** Ribosomal protein L5 |
ENSGALG00000005922 | F: 5'-AATATAACGCCTGATGGGATGG-3' R:5'-CTTGACTTCTCTCTTGGGTTTCT-3’ | 99 |
|
RPL30 Ribosomal protein 30 |
ENSGALG00000029897 | F: 5'-ATGATTCGGCAAGGCAAAGC-3' R: 5'-GTCAGAGTCACCTGGGTCAA |
273 |