Figure 7.

Heterotypic interaction following semaphorin 3A stimulation.A, fraction correlated after stimulation. No apparent increase is observed like that of plexin D1–Nrp1 following semaphorin 3C stimulation. Gray numbers above each column represent the number of single cells analyzed. B, changes in average f_c value following stimulation. Plexin A2–Nrp1 and plexin A4–Nrp1 have significant increases in correlation, whereas the plexin D1–Nrp1 interaction is unchanged. C, diffusion change for plexin A4-eGFP and plexin A2-eGFP when coexpressed with Nrp1 and stimulated with semaphorin 3A. Both plexin A4 and plexin A2 have significantly decreased average diffusion coefficients adding to evidence that a weak/transient interaction is formed. f_c, fraction of cross-correlation; Nrp1, neuropilin-1.