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. 2021 Jul 13;297(2):100947. doi: 10.1016/j.jbc.2021.100947

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

A dominant negative Zbtb2 construct stabilizes the interaction with the Ep400 complex.A, Nanog>GFP intensities after switching from 2iLIF to N2B27 +CHIR +bFGF in the presence of DOX of indicated genotypes. B, volcano plot of protein enrichments in AP-MS of mESCs overexpressing ZBTB2-HAF compared with those overexpressing ZBTB2-AVI. ZBTB2 and partner TFs are indicated in blue, NuRD subunits in red, HiRA subunits in green, and Ep400 subunits in orange. AP-MS, affinity purification–MS; bFGF, basic FGF; CHIR, CHIR99021; HiRA, histone regulator A; N2B27, chemically defined medium; NuRD, nucleosome remodeling and deacetylase; TFs, transcription factors.