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. 2021 Jul 26;12:704550. doi: 10.3389/fneur.2021.704550

Figure 5.

Figure 5

Effects of antagomir 223 on hippocampal miR 223 levels and seizure-like EEG findings. Mice were injected intracerebroventricularly with antagomir 223 or antagomir-NC alone or at 60 min after status epilepticus. (A) In situ hybridization of miR-223 in the hippocampal tissue. High expression levels of miR-223 were observed in the CA1 and CA3 regions of the KA and KA+Antagomir-NC groups, while the miR 223 hybridization signals in these regions were weaker in the KA+Antagomir-223 group. Scale bar = 100 μm. (B,C) OD analysis revealed higher expression levels in the CA1 and CA3 regions of KA-treated mice relative to control. In the KA+Antagomir-223 group, the expression of miR-223 was reduced in the CA1 and CA3 regions (n = 3 per group). (D) The hippocampal tissues were analyzed to assess the miR-223 levels using qRT-PCR (n = 6 per group). (E) Representative EEG images of mice, taken 15 min after the final injection. (F) The number of seizure spikes in EEG. Statistically significant differences were determined using the unpaired t-test. Data are presented as means ± SD; *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant. EEG, electroencephalography; KA, kainic acid; OD, optical density; ns, not significant; SD, standard deviation.