Fig. 2.

Proteome characterization of SCC-9-and LN1-derived EVs. The overlap between proteins identified for EVs isolated from primary tumor and lymph node metastatic cells is shown in the Venn diagram (A). Unsupervised hierarchical cluster heat map (B) and PCA plot (C) show grouping of replicates according to the proteomic profile. The cluster was generated using the Euclidean distance and complete linkage method (n = 1722 proteins). The top ten GO biological processes and subcellular location enriched for the differentially abundant proteins between the two groups are shown in panels D and E, respectively. ∗p-value ≤ 0.05; ∗∗p-value ≤ 0.01; ∗∗∗p-value ≤ 0.001 (p-values were determined using the Bonferroni method). EVs, extracellular vesicles; GO, Gene Ontology; PCA, principal component analysis.