Fig. 3.

miRNA, metabolomics, and lipidomics analysis of EVs isolated from SCC-9 and LN1 cell lines. Sample grouping according to miRNA, metabolite, and lipid profiles are shown in the dendrograms (upper panel) and PCA plots (lower panel) for SCC-9 EV and LN1 EV replicates (A, C, and E, respectively). The dendrograms were generated using Euclidean distance and complete linkage method (n = 457 miRNAs, 33 metabolites, and 153 lipids). Differentially expressed miRNAs can target specific genes that are significantly mapped to KEGG pathways (B; p-value ≤ 0.05). Differentially expressed metabolites are associated with specific biological processes (D; p-value ≤ 0.05). The class composition of differentially abundant lipids between SCC-9 and LN1-derived EVs is shown in panel F. EVs, extracellular vesicles; KEGG, Kyoto Encyclopedia of Genes and Genomes.