Table 1.
List of PCR Primers
| Genes | Primer sequence, 5′-3′ |
|
|---|---|---|
| Forward | Reverse | |
| hβ-actin | GGACTTCGAGCAAGAGATGG | AGCACTGTGTTGGCGTACAG |
| hClaudin-1 | CGATGAGGTGCAGAAGATGA | CCAGTGAAGAGAGCCTGACC |
| hClaudin-2 | TTGTGACAGCAGTTGGCTTC | TCATGCCCACCACAGAGATA |
| hClaudin-4 | AAGTGACAGGGTGTGGTGGT | TACCCGGAACAGAGGAGATG |
| hClaudin-7 | AATTTTCATCGTGGCAGGTC | GGACAGGAACAGGAGAGCAG |
| hJAM-A | AGCCATCAGTCAAGGGTCAC | GAGGCGGAGGTTACAGTGAG |
| hOccludin | TATGGAGGAAGTGGCTTTGG | ATGCCCAGGATAGCACTCAC |
| hZO-1 | CAGCAACTTTCAGACCACCA | GTGCAGTTTCACTTGGCAGA |
| hLPA5R | ATCTTCCTGCTGTGCTTCGT | CCCTCGGCGCTAAAGTAGTA |
| mβ-actin | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
| mClaudin-1 | AAGGGTGTTGCCATTGACAT | TGCAACATAGGCAGGACAAG |
| mClaudin-2 | AAGGTGCTGCTGAGGGTAGA | TTGAGCATTCAAAGCACAGG |
| mClaudin-4 | TGGAACCCTTCCGTTGATTA | CACTGGGCTGCTTCTAGGTC |
| mClaudin-7 | CTGGTGTTGGGCTTCTTAGC | TGATGACCAATCCAGGAACA |
| mJAM-A | AGCCAGATCACAGCTCCCTA | GACAGAGGAGGGGACACTGA |
| mOccludin | AGTGGGTCAGGGAATATCCA | TCAGCAGCAGCCATGTAACTC |
| mZO-1 | GTCCCTGTGAGTCCTTCAGC | GAAGGGCTCCTTGTGGGATA |
| mTNF-α | TCGTAGCAAACCACCAAGTG | AGATAGCAAATCGGGTGACG |
| mIFN-γ | TCTCCAGAAACCCTCACTGGT | TCAGCGGATTCATCTGCTTCG |
| mIL-1β | TGAAATGCCACCTTTTGACA | GTAGCTGCCACAGGCTTCTCC |
| mIL-6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
| mLPA5R | GCTCCAGTGCCCTGACTATC | GGGAAGTGACAGGGTGAAGA |