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. 2021 Jul 26;9:707797. doi: 10.3389/fchem.2021.707797

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Copyright © 2021 Cooper, Bird, Sheng, Choi, Silkstone, Simons, Syrett, Piano, Ronda, Bettati, Paredi, Mozzarelli and Reeder.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of GSH on stability of Hb-PEG conjugates. SDS-PAGE comparing stability of A12 conjugation with maleimide-PEG and mono-sulfone PEG following incubation for 7 days at 37°C with PBS or 1 mM GSH. Labels equate to % of total protein in lane in each band for: Di-PEGylated Hb monomer; Mono-PEGylated Hb Monomer; Hb dimer; Hb monomer.