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. 2021 Jul 26;9:707797. doi: 10.3389/fchem.2021.707797

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Copyright © 2021 Cooper, Bird, Sheng, Choi, Silkstone, Simons, Syrett, Piano, Ronda, Bettati, Paredi, Mozzarelli and Reeder.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kinetics of stability of A12 Hb-PEG conjugates. Graphs show the % drop in Hb PEGylation (assuming 100% PEGylation at t = 0). Curve fits illustrated are mono exponential decay for the maleimide-PEG species. The mono-sulfone-PEG curve fits superimpose on the maleimide-PEG + PBS fit so have been removed for clarity. Legend: + PBS maleimide-PEG (o); + PBS mono-sulfone-PEG (□); + 1 mM GSH maleimide-PEG (●), + 1 mM GSH mono-sulfone PEG (■).