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. 2021 Jul 26;11:676456. doi: 10.3389/fcimb.2021.676456

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Copyright © 2021 Sengupta, Nayak, Meuli, Sander, Mishra and Sonawane

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Role of MAPK in H3K9 hypermethylation and autophagy inhibition. Expression of G9a transcripts was checked in RAW264.7 cells infected with Msm_pSMT3and Mtb_Prtfollowed by treatment with (A) SB203580 and (B) U0126 for 24 h. Expression of (C) p-P38, (D) ATG5 and ATG7, (E) LC3-I to II conversion (Densitometry is representative to the particular western blot data), (F) H3K9me2/3, (G) H3K9ac and HDAC3 were checked in RAW cells infected with Msm_pSMT3and Mtb_Prt in presence and absence of SB203580 (P38 inhibitor) for 24 h. The experiments were performed in triplicate (n=3). Msm_pSMT3- Msm harbouring pSMT3 plasmid; Mtb_Prt- recombinant Msm expressing MtbPrt (Rv3242c). Results are shown as mean ± S.D. ***p < 0.001; ns, not significant.