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. 2021 Aug 4;14:3471–3483. doi: 10.2147/DMSO.S307771

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© 2021 Hu et al.

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MiR-29b repressed the expression of SERPINH1 in HRECs. (A) The predicted complementary sequences between miR-29b and SERPINH1 3ʹUTR. (B and C) The luciferase reporter activities of SERPINH1 3ʹUTR-wt (B) and SERPINH1 3ʹUTR-mut (C) in HRECs after being transfected with mimics NC or miR-29b mimics were determined by Dual-Luciferase Reporter assay. (D) The miR-29b expression level in HRECs after being transfected with mimics NC or miR-29b mimics were determined by qRT-PCR. (E and F) The mRNA and protein expression levels of SERPINH1 in HRECs after being transfected with mimics NC or miR-29b mimics were determined by qRT-PCR. (G) The miR-29b expression level in HRECs after being transfected with inhibitor NC or miR-29b inhibitor was determined by qRT-PCR. (H and I) The mRNA and protein expression levels of SERPINH1 in HRECs after being transfected with inhibitor NC or miR-29b inhibitor were determined by qRT-PCR. (J) The mRNA expression levels of miR-29b in the HRECs after different treatments were determined by qRT-PCR. *p<0.05, **p<0.01 and ***p<0.001.