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. 2021 Aug 4;14:3471–3483. doi: 10.2147/DMSO.S307771

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© 2021 Hu et al.

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MiR-29b/SERPINH1 axis participated in the glucose-induced increase in HREC proliferation and migration. (A) The mRNA expression level of SERPINH1 in HRECs after being transfected with pcDNA3.1 or pcDNA3.1-SERPINH1 was determined by qRT-PCR. (B–D) HRECs were transfected with pcDNA3.1 or pcDNA3.1-SERPINH1, the cell viability, proliferation and migration of HRECs were determined by MTS (B), EdU (C) and wound healing assay (D), respectively. (E–G) HRECs were transfected with different miRNAs or plasmids followed by treatment with 25 mM glucose, the cell viability, proliferation and migration of HRECs were determined by MTS, EdU and wound healing assay, respectively. *p<0.05, **p<0.01 and ***p<0.001.