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. 2021 Jul 26;12:684903. doi: 10.3389/fmicb.2021.684903

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Copyright © 2021 Miao, Wang, Adyel, Zhao, Yan, Wu and Hou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Relative abundance of the top 10 most abundant metabolic function pathways in bacterial biofilms from artificial (ACF and PVC) and natural (pebble and wood) substrates. Statistical comparison between the substrate types was performed using one-way ANOVA followed by Tukey’s post-hoc tests, and results FIGURE 5 | are provided in Supplementary Table S13. (B) Heatmap-mean analysis of bacterial biofilms at the function level (n = 6). (C) Principal component analysis (PCA) plot depicts bacterial biofilms on artificial (ACF and PVC) and natural (pebble and wood) substrates based on the Bray-Curtis distance. Statistical comparison was deduced by PERMANOVA, and results are provided in Supplementary Table S14. (D) Comparison of the bacterial metabolic function pathways’ abundance between artificial (ACF and PVC) and natural (pebble and wood) substrates using Statistical Analysis of Metagenomic Profiles (STAMP). See also Supplementary Table S15.