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. 2021 Jul 26;9:685363. doi: 10.3389/fcell.2021.685363

TABLE 1.

The gene-specific primers for the q-PCR.

Target genes Forward (5′–3′) Reverse (5′–3′)
Lgr5 CGGGACCTTGAAGATTTCCT GATTCGGATCAGCCAGCTAC
Olfm4 CGAGACTATCGGATTCGCTATG TTGTAGGCAGCCAGAGGGAG
Tnfrsf19 CTTCCGTGACAGCATTTACCTT CTGCTCAGTGAAGCCATAGGG
Smoc2 CCCAAGCTCCCCTCAGAAG GCCACACACCTGGACACAT
Wnt3 GGGCCAGCAGTACACATCTC TCACACCTTCTGCTACGCTG
β-catenin TGGACGTGGGCGAACTTTTA GCGTTCTCGAGGACCAGTTT
Ascl2 GCCTGACCAAATGCCAAGTG ATTTCCAAGTCCTGATGCTGC
Axin2 GCTCCAGAAGATCACAAAGAGC AGCTTTGAGCCTTCAGCATC
Cd44 CCTTGGCCACCACTCCTAAT TGGGAGTCTTCACTTGGGGTA
EphB2 TCTATGTCTTCCAGGTGCGG TGGTCTGGTACTCGGCTTCT
Hey1 AACGACATCGTCCCAGGTTT ATTGATTCGGTCTCGTCGGC
Hes1 TCAACACGACACCGGACAAA ATTCTTGCCCTTCGCCTCTT
Dll1 GGTTTGTGTGTGACGAGCAC ATCTTCTCCCCTCTGTCCCC
Dll4 CCAGCAACCCCTGTCGAAAT ACAGTGCTGGCCATAGTAGC
Reg3b ACTCCCTGAAGAATATACCCTCC CGCTATTGAGCACAGATACGAG
Reg3g ATGCTTCCCCGTATAACCATCA GGCCATATCTGCATCATACCAG