TABLE 1.
Antibody | Epitopes | mAb/pAb | WB | IHC or IF | Source |
α-tubulin | α-tubulin | mAb | 1:2000 | Abcam | |
Iba1 | ionized calcium binding adapter molecule 1 | pAb | 1:200 | Wako | |
t-GSK3β | total GSK3β | pAb | 1:1000 | Cell Signaling | |
p-GSK3β | p-GSK3β at Ser9 | pAb | 1:1000 | Cell Signaling | |
t-Nrf2 | total Nrf2 | pAb | 1:500 | Abcam | |
p-Nrf2 | p-Nrf2 at Ser40 | pAb | 1:1000 | 1:100 | Abcam |
5-LO | total 5-lipoxygenase | mAb | 1:1000 | 1:100 | Abcam |
NF-κB p65 | total NF-κB p65 | mAb | 1:500 | Cell Signaling | |
GAPDH | full length GAPDH | mAb | 1:1000 | Abcam | |
Histone3 | total histone H3 protein | pAb | 1:1000 | Cell Signaling |
p, phosphorylated; mAb, monoclonal antibody; pAb, polyclonal antibody; WB, Western blotting; IHC, immunohistochemistry; IF, immunofluorescence.