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. 2021 May 5;12(3):1121–1143. doi: 10.1016/j.jcmgh.2021.04.016

Figure 2.

Figure 2

Function of TAK1 in HCC. (A) Proportion of annexin V-stained apoptotic cells analyzed by flow cytometry in Huh7 or HCCLM3 cells transfected with TAK1 siRNA or OXO treatment, a TAK1 inhibitor. Three biological repeats per group. (B) Cell cycle analysis and representative results of HCCLM3 cells using flow cytometry on OXO treatment. Three biological repeats per group. (C) Colony formation of HCCLM3 cells treated with different concentration of OXO. Colony numbers were measured by Image J software. Three biological repeats per group. (D) Colony formation of different HCC cell lines on OXO treatment with different concentrations. (E) Real-time (RT) qPCR quantification of Bcl-2 and GADD45β mRNA in HCCLM3 cells treated with OXO or not. (F and G) Western blotting analysis of TAK1 downstream proteins levels on genetic knockdown or pharmacologic inhibition of TAK1 in Huh7 and HCCLM3 cells.