Fig. 2.

INSM1 localization and Cyclin D1 expression varies depending on the islet genomic profile. (A) Representative images of INSM1 and Cyclin D1 immunohistochemical staining in wild-type, Phlda3^−/− and Men1^−/− male mice of 45 weeks. (B) Graph showing Cyclin D1 staining intensities in mice islets, quantified using ImageJ. Values represent means of data obtained from three mice ± SD. The relative intensity of the staining was calculated fractioning the integrated density by the area. (C) Representative images of hematoxylin & eosin, INSM1 and Cyclin D1 immunohistochemical staining in Men1^−/- mice islets. (D) Graph showing Cyclin D1 staining intensities in mice islets, quantified using ImageJ. Values represent means of data obtained from three mice ± SD. ***P-value < 0.001 as determined by t test.