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. 2021 Jul 26;14:682320. doi: 10.3389/fnmol.2021.682320

FIGURE 2.

FIGURE 2

Lovastatin attenuates α-syn phosphorylation in GFP-α-syn-HEK293 cells. (A) Immunofluorescence showing that p-S129 co-localized with α-syn aggregates. Intracellular p-S129 α-syn (red) was decreased in the lovastatin group. (B) Quantitative analysis of p-S129 α-syn fluorescence. (C) Western blot analysis of the p-S129 level. (D) Quantification of the p-S129 level in Western blot. Results are normalized to total α-syn. (E) Western blot analysis of soluble and insoluble p-S129 α-syn. (F) Quantification of soluble p-S129 α-syn. Results are normalized to total α-syn. (G) Quantification of insoluble p-S129 α-syn. Results are normalized to total α-syn. All data are means ± SEM, **P < 0.01, ***P < 0.001, and ****P < 0.0001, ns: not statistically significant, one-way ANOVA with Tukey’s multiple comparisons test. Bar = 20 μm. All experiments were performed in triplicate for at least three independent times.