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. 2021 Jul 26;8:704685. doi: 10.3389/fmolb.2021.704685

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Copyright © 2021 Siukstaite, Imberty and Römer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Structure of the glycosphingolipid Gb3. (B) StxB- and LecA-induced membrane invaginations on Gb3-containing GUVs. (C) Endocytotic pathways of StxB and LecA. (D) Different effects of lectin topology (LecA and StxB) in proto-tissue (3D assembly of giant liposomes) formation (Parts of panel D adapted from image by Sarah Villringer and licensed under CC BY 4.0; https://doi.org/10.1038/s41598-018-20230-6). AF488 (Alexa Fluor 488) and TxRed (Texas Red) are fluorophors, and DHPE-TxRed represents Texas Red linked to the phospholipid 1,2-Dihexadecanoyl- sn -Glycerol -3-Phosphoethanolamine. All scale bars are 10 μM, and schematic representations were made using Servier Medical Art (https://smart.servier.com/).