Figure 4.

miR-4277 represses the expression of CYP3A4 in MHCC97-H cells. The effect of miR-4277 on wild type CYP3A4 or CYP3A4 mutations (CYP3A4Mut1 [the vector of cyp3a4 with the mutation of the 1^st miR-4277 targeting site], CYP3A4^Mut2 [the vector of cyp3a4 with the mutation of the 2^nd miR-4277 targeting site], or CYP^Mut [the vector of cyp3a4 with the mutation of the 1^st and 2^nd miR-4277 targeting site]) in MHCC97-H cells was measured by western blot. The results are shown as blots (A) or the quantitative results (B). *P < 0.05.