Table 2.
Extract yields and GZA-amount recoveries in different individual solvents with and W/O the use of temperatures.
| Sample # | US factors values tested |
Temperature (°C) | Extract yield (mg/1mg) in different solvents |
Solvent average yield and sum (SD) |
Range | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Time (min) | Amplitude (%) | Pulse (seconds) | Particle size (mm) | AC | EtOH | H2O | ||||||
| 1–27 | 1 | 30 | 10/0.5 | 0.5 | 0 | 0.03 | 0.02 | 0.06 | AC | Sum | 1.82 | 0.03–0.38 |
| 2 | 40 | 20/0.5 | 0.05 | 0.04 | 0.06 | Mean | 0.20 | |||||
| 3 | 50 | 30/0.5 | 0.11 | 0.04 | 0.12 | SD | 0.13 | |||||
| 1 | 30 | 10/0.5 | 1 | 0.10 | 0.03 | 0.19 | EtOH | Sum | 0.52 | 0.02–0.15 | ||
| 2 | 40 | 20/0.5 | 0.27 | 0.05 | 0.26 | Mean | 0.06 | |||||
| 3 | 50 | 30/0.5 | 0.34 | 0.07 | 0.41 | SD | 0.04 | |||||
| 1 | 30 | 10/0.5 | 1.5 | 0.24 | 0.05 | 0.29 | H2O | Sum | 2.21 | 0.06–0.43 | ||
| 2 | 40 | 20/0.5 | 0.30 | 0.07 | 0.39 | Mean | 0.25 | |||||
| 3 | 50 | 30/0.5 | 0.38 | 0.15 | 0.43 | SD | 0.15 | |||||
| 28–54 | 1 | 30 | 10/0.5 | 0.5 | 20 | 0.06 | 0.04 | 0.09 | AC | Sum | 2.10 | 0.06–0.41 |
| 2 | 40 | 20/0.5 | 30 | 0.08 | 0.09 | 0.12 | Mean | 0.23 | ||||
| 3 | 50 | 30/0.5 | 40 | 0.13 | 0.14 | 0.14 | SD | 0.13 | ||||
| 1 | 30 | 10/0.5 | 1 | 20 | 0.17 | 0.08 | 0.21 | EtOH | Sum | 1.49 | 0.04–0.32 | |
| 2 | 40 | 20/0.5 | 30 | 0.33 | 0.19 | 0.35 | Mean | 0.17 | ||||
| 3 | 50 | 30/0.5 | 40 | 0.36 | 0.24 | 0.41 | SD | 0.09 | ||||
| 1 | 30 | 10/0.5 | 1.5 | 20 | 0.21 | 0.17 | 0.22 | H2O | Sum | 2.41 | 0.09–0.48 | |
| 2 | 40 | 20/0.5 | 30 | 0.35 | 0.22 | 0.39 | Mean | 0.27 | ||||
| 3 | 50 | 30/0.5 | 40 | 0.41 | 0.32 | 0.48 | SD | 0.14 | ||||
| GZA-amount (μg/mg) in different solvents | ||||||||||||
| AC | EtOH | H2O | ||||||||||
| 1–27 | 1 | 30 | 10/0.5 | 0.5 | 0 | 0.50 | 0.80 | 2.08 | AC | Sum | 6.26 | 0.50–0.87 |
| 2 | 40 | 20/0.5 | 0.78 | 1.48 | 3.82 | Mean | 0.70 | |||||
| 3 | 50 | 30/0.5 | 0.80 | 1.78 | 5.59 | SD | 0.25 | |||||
| 1 | 30 | 10/0.5 | 1 | 0.78 | 1.11 | 2.53 | EtOH | Sum | 14.90 | 0.80–2.54 | ||
| 2 | 40 | 20/0.5 | 0.80 | 1.72 | 4.86 | Mean | 1.66 | |||||
| 3 | 50 | 30/0.5 | 0.85 | 2.00 | 5.63 | SD | 0.51 | |||||
| 1 | 30 | 10/0.5 | 1.5 | 0.10 | 1.55 | 2.70 | H2O | Sum | 41.29 | 2.08–7.56 | ||
| 2 | 40 | 20/0.5 | 0.79 | 1.93 | 6.51 | Mean | 4.59 | |||||
| 3 | 50 | 30/0.5 | 0.87 | 2.54 | 7.56 | SD | 1.92 | |||||
| 28–54 | 1 | 30 | 10/0.5 | 0.5 | 20 | 1.33 | 1.35 | 2.56 | AC | Sum | 23.88 | 1.33–4.82 |
| 2 | 40 | 20/0.5 | 30 | 1.59 | 1.66 | 3.12 | Mean | 2.65 | ||||
| 3 | 50 | 30/0.5 | 40 | 3.95 | 2.01 | 5.96 | SD | 1.45 | ||||
| 1 | 30 | 10/0.5 | 1 | 20 | 1.33 | 1.80 | 2.57 | EtOH | Sum | 19.50 | 1.35–3.48 | |
| 2 | 40 | 20/0.5 | 30 | 2.07 | 2.17 | 5.83 | Mean | 2.17 | ||||
| 3 | 50 | 30/0.5 | 40 | 4.61 | 2.30 | 5.15 | SD | 0.61 | ||||
| 1 | 30 | 10/0.5 | 1.5 | 20 | 1.41 | 2.29 | 3.07 | H2O | Sum | 43.31 | 2.56–8.23 | |
| 2 | 40 | 20/0.5 | 30 | 2.77 | 2.44 | 6.82 | Mean | 4.81 | ||||
| 3 | 50 | 30/0.5 | 40 | 4.82 | 3.48 | 8.23 | SD | 2.07 | ||||