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. 2021 Aug 6;41(8):BSR20211238. doi: 10.1042/BSR20211238

Figure 4. ELISA for the detection of SARS-CoV-2 antibodies in clinical serum samples.

Figure 4

(A,B) Coating of 96-well ELISA plate by optimized concentration of SARS-CoV-2 antigen followed by blocking. (C,D) Clinical serum samples added to the plate and allowed to incubate followed by addition of horseradish peroxidase (HRP)-labeled secondary antibody (Ab). (E) Addition of substrate. (F) Formation of a chromogenic product. (G) The intensity of the chromogenic product formed measured using an ELISA plate reader and further analysis of the concentration of SARS-CoV-2 Abs present in the clini cal serum samples.