Table 1.
Validation parameters of the method for the determination of the enrofloxacin (ENR), ciprofloxacin (CIP) and lincomycin (LIN) in whole eggs/egg yolks/egg whites.
| Analyte | Matrix | Repeatability*, (CV,%) |
Within-lab Reproducibility*, (CV,%) |
LOQ (µg/kg) |
LOD (µg/kg) |
Recovery* (%) |
|---|---|---|---|---|---|---|
| ENR | Whole egg | 7.3 ± 1.9 | 12.2 ± 4.3 | 1.00 | 0.50 | 102.4 ± 5.6 |
| Egg yolk | 10.0 ± 2.4 | 14.3 ± 5.5 | 1.00 | 0.50 | 89.0 ± 4.1 | |
| Egg white | 6.3 ± 2.3 | 12.9 ± 4.1 | 1.00 | 0.50 | 96.0 ± 4.4 | |
| CIP | Whole egg | 8.8 ± 2.5 | 13.1 ± 4.2 | 1.00 | 0.50 | 91.6 ± 5.2 |
| Egg yolk | 9.7 ± 2.7 | 14.8 ± 6.1 | 1.00 | 0.50 | 111.0 ± 5.8 | |
| Egg white | 7.1 ± 1.8 | 11.9 ± 5.3 | 1.00 | 0.50 | 86.0 ± 3.8 | |
| LIN | Whole egg | 6.4 ± 1.7 | 13.3 ± 3.8 | 1.00 | 0.50 | 90.7 ± 4.1 |
| Egg yolk | 9.4 ± 2.1 | 14.4 ± 5.7 | 1.00 | 0.50 | 87.0 ± 4.4 | |
| Egg white | 5.3 ± 1.9 | 11.6 ± 5.1 | 1.00 | 0.50 | 89.0 ± 5.2 |
Abbreviations: LOD, limit of detection; LOQ, limit of quantification.
⁎
Average of 3 validation levels with standard deviation (± SD).