KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| V5 | Invitrogen | R96025 |
| V5/HRP | Invitrogen | R961-25 |
| SMAD4 | Santa Cruz Biotechnology | B-8; sc-7966 |
| beta-actin/HRP | Santa Cruz Biotechnology | sc-47778 HRP |
| TRK | Cell Signaling Technology | A7H6R; #92991 |
| FOSL1 | Cell Signaling Technology | D80B4, #5281 |
| SMAD4 | Cell Signaling Technology | D3M6U, #38454 |
| Histone H3 Ac-K27 | AbCam | ab4729 |
| SMAD1 | Santa Cruz | sc-7965 |
| Phospho-Smad1/5/8 | Cell Signaling Technology | 41D10 |
| Phospho-SMAD2 | Cell Signaling Technology | 3101S |
| GAPDH | Cell Signaling Technology | 14C10 |
| Bacterial and virus strains | ||
| Metastasis Candidate ORF - Pool1 | Broad Institute | This manuscript |
| Metastasis Candidate ORF - Pool2 | Broad Institute | This manuscript |
| Metastasis Candidate ORF - Pool3 | Broad Institute | This manuscript |
| Chemicals, peptides, and recombinant proteins | ||
| Blasticidin | ThermoFisher Scientific | A1113903 |
| Puromycin | ThermoFisher Scientific | A1113803 |
| Hygromycin | Life Technologies | 10687010 |
| Doxycycline | Clontech | 3P 631311 |
| Recombinant TGFβ | Peprotech | AF-100-21C |
| Critical commercial assays | ||
| QiaAmp DNA Blood Maxi Kit | QIAGEN | 51194 |
| DNeasy Blood and Tissue kit | QIAGEN | 69506 |
| RNAlater-ICE Frozen Tissue Transition Solution | ThermoFisher | AM7030 |
| Phusion high-fidelity DNA polymerase | New England Biolabs | M0530 |
| TaqMan Universal PCR Master Mix | ThermoFisher | 4364338 |
| RNeasy Plus Mini kit | QIAGEN | 74134 |
| High-Capacity cDNA Reverse Transcription Kit | ThermoFisher | 4368813 |
| Power SYBR Green PCR Master Mix | Invitrogen | 4367659 |
| Deposited data | ||
| SMAD4 RNA-seq | This manuscript | GSE148248 |
| Experimental models: Cell lines | ||
| Lenti-X 293T | Takara | 632180 |
| PANC1 | Broad Institute | Project Achilles |
| PATU8902 | Broad Institute | Project Achilles |
| HPAC | Broad Institute | Project Achilles |
| MiaPaCa2 | Broad Institute | Project Achilles |
| L3.3 | Broad Institute | Project Achilles |
| PANC0327 | Broad Institute | Project Achilles |
| Experimental models: Organisms/strains | ||
| CrTac:NCr-Foxn1nu | Taconic | NCRNU-F |
| Recombinant DNA | ||
| pXPR003-sgCTRL | Broad Institute | This study |
| pXPR003-sgSMAD4 | Broad Institute | This study |
| pLX307-luciferase | Broad Institute | This study |
| pLX307-SMAD4 | Broad Institute | This study |
| pXPR003-sgGFP | Broad Institute | This study |
| PLKO shFOSL1-1 | Broad Institute | This study |
| PLKO shFOSL1-2 | Broad Institute | This study |
| FUW-LacZ-Luciferase-mCherry | This manuscript | This study |
| FUW-SMAD4-Luciferase-mCherry | This manuscript | This study |
| Software and algorithms | ||
| Tophat 2.0.2 | NA | Ghosh and Chan, 2016 |
| HTseq | NA | Anders et al., 2015 |
| DEseq | NA | Love et al., 2014 |