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. 2021 Jul;9(14):1148. doi: 10.21037/atm-21-2589

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Graphical representation of MDSCs definition by flow cytometry. To determine the percentage of MDSC subsets in patients, CD11b⁺CD33⁺ myeloid-derived cell populations were first gated using FITC-anti-CD33 and PE-anti-CD11b antibodies. The M-MDSCs were stained with PE-cy7-CD14 and APC-HLA-DR antibodies while PE-cy7-CD14 and APC-CD15 antibodies for PMN-MDSCs. MDSCs, myeloid-derived suppressor cells; M-MDSCs, monocytic myeloid-derived suppressor cells; PMN-MDSCs, polymorphonuclear myeloid-derived suppressor cells.