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. 2021 Jul;9(14):1148. doi: 10.21037/atm-21-2589

Figure 5.

Figure 5

Isolated CD73+ MDSCs from cancer patients inhibits CD3+ T-cell proliferation. (A) Purified CD3+ T cells (T) were stimulated by anti-CD3/anti-CD28 in the presence of indicated MDSCs subsets (M for M-MDSCs and P for PMN-MDSCs) from peripheral blood of HNSCC patients at M:T ratios of 2:1 and 4:1 for 3 d (n=4). (B) Purified MDSCs subsets were incubated at a ratio of 2:1 or 4:1 with autologous CD3+ T cells in the presence of CD73 inhibitors APCP (A) for 3 d. The production of IFN-γ by CD8+ T cells were determined and summarized by flow cytometry (n=4). * indicates a P value of <0.05; ** indicates a P value of <0.01; *** indicates a P value of <0.0001. MDSCs, myeloid-derived suppressor cells; M-MDSCs, monocytic myeloid-derived suppressor cells; PMN-MDSCs, polymorphonuclear myeloid-derived suppressor cells; HNSCC, head and neck squamous cell carcinoma; APCP, adenosine 5'-(α,β-methylene)diphosphate; IFN-γ; interferon-γ.