FIGURE 1.

(A) Map of core-carbon metabolism in the engineered Z. mobilis strain. A list of abbreviations used are shown in Supplementary Table 1. (B) Kinetic properties of glucose facilitator (glf) in Z. mobilis BC42C Δpdc. Kinetic parameters (V_max, K_m, and K_i) are estimated by fitting the time-course utilization of glucose (green) and xylose (blue) with coefficient of determination R² = 0.99. Dots denote experimental data points, and lines denote the best fit curve of substrates uptake kinetics. (C,D) Contour plots illustrating substrate uptake rates as a function of glucose (C) and xylose (D) concentrations.