Figure 1. Epithelial CRAMP production is defective in dietary gluten‐induced enteropathy (GIE).

• A
  Animal protocol.
• B
  Serum CRAMP determination by ELISA (n = 8).
• C, D
  Western blot of pro‐form CRAMP and mature peptide in duodenum and ex vivo duodenal epithelial cells.
• E
  Localization and expression of CRAMP (red), E‐cadherin (green), F4/80 (green) and Ly6G (green) in duodenum by immunofluorescent staining. Representative photomicrographs of individual and merged staining were shown. Nuclei were stained with DAPI (blue). Scale bar: 50 μm.

Data information: Data in B were representative and were the mean ± SD from three independent experiments. Data (C–E) were representative from three independent experiments. P values were calculated by unpaired two‐tailed t‐test for comparison of two groups.

Source data are available online for this figure.