Figure EV1. The production of cathelicidin (CRAMP in mice and LL‐37 in humans) in mice and in human subjects. Related to Fig 1.

1. Serum CRAMP determination by ELISA. Rag1 ^−/−/gluten‐free: unsensitized Rag1 ^−/−mice fed with gluten‐free diet. Rag1 ^−/−/gluten: unsensitized Rag1 ^−/− mice fed with gluten‐containing diet (n = 8).
2. Western blot of pro‐form CRAMP and the mature peptide in duodenum of Rag1 ^−/−, Cnlp ^−/− Rag1 ^−/−, C57BL/6 and BALB/c mice.
3. Serum LL‐37 determination by ELISA. Anti‐tTG IgA: anti‐tissue transglutaminase immunoglobulin A antibodies. Anti‐DGP IgG: anti‐deamidated gliadin peptides immunoglobulin G antibodies (Anti‐tTG IgA⁻Anti‐DGP IgG⁻, n = 50; Anti‐tTG IgA⁺Anti‐DGP IgG⁻, n = 46; Anti‐tTG IgA⁻Anti‐DGP IgG⁺, n = 50; Anti‐tTG IgA⁺Anti‐DGP IgG⁺, n = 13).

Data information: Data (A, C) were representative and were the mean ± SD from three independent experiments. Data in B were representative from three independent experiments. P values were calculated by unpaired two‐tailed t‐test for comparison of two groups (A) or one‐way ANOVA followed by Tukey’s post hoc test for multiple comparisons (C).

Source data are available online for this figure.