| Reagent/Resource | Reference or Source | Identifier or Catalog Number |
|---|---|---|
| Experimental Models | ||
| Mouse: C57BL/6J: | GemPharmatech | C57BL/6JGpt; Cat# N000295; RRID: SCR_017239 |
| Mouse: Cnlp −/−: | Jackson Laboratory | B6.129X1‐Camptm1Rlg/J Mus musculus; Cat# JAX:017799; RRID: IMSR_JAX:017799 |
| Mouse: Rag1 −/−: | GemPharmatech | B6/JGpt‐Rag1em1Cd /Gpt; Cat# T004753; RRID: SCR_017239 |
| Mouse: Cnlp −/− Rag1 −/− | This study | N/A |
| Cell lines: Caco‐2 | ATCC | Cat# HTB‐37; RRID: CVCL_0025 |
| Patient samples: Human serum | Second Affiliated Hospital of Nanchang University | Ethics number: no. [2010] 041 |
| Bacterial strains: P. aeruginosa PAO1 | ATCC | Cat# 47085 |
| Bacterial strains: P. aeruginosa PA14 | Isolated from patient | N/A |
| Antibodies | ||
| Anti‐CRAMP (1‐39) antibody | Innovagen | Cat# PA‐CRPL‐100 |
| Anti‐claudin‐1 antibody | Abcam | Cat# ab180158 |
| Anti‐occludin antibody | Abcam | Cat# ab216327; RRID: AB_2737295 |
| Anti‐zonulin antibody | Abcam | Cat# ab131236; RRID: AB_11157376 |
| Anti‐TRAF6 antibody | Abcam | Cat# ab40675; RRID: AB_778573 |
| Anti‐p65 antibody | Abcam | Cat# ab32536; RRID: AB_776751 |
| Anti‐p‐AKT antibody | Cell Signaling Technology | Cat# 4060; RRID: AB_2315049 |
| Anti‐AKT antibody | Cell Signaling Technology | Cat# 4685; RRID: AB_2225340 |
| Anti‐MyD88 antibody | Cell Signaling Technology | Cat# 4283; RRID: AB_10547882 |
| Anti‐p‐p65 antibody | Cell Signaling Technology | Cat# 3033; RRID: AB_331284 |
| Anti‐ZO‐1 antibody | Thermo Fisher Scientific | Cat# 40‐2200; RRID: AB_2533456 |
| Anti‐ZO‐2 antibody | Thermo Fisher Scientific | Cat# 71‐1400; RRID: AB_88012 |
| Anti‐ACTB antibody | ABclonal | Cat# AC026; RRID: AB_2768234 |
| Alexa Fluor® 700 anti‐mouse CD45 | Biolegend | Cat# 103128; RRID: AB_493715 |
| Brilliant Violet® 711 anti‐mouse CD11c | Biolegend | Cat# 117349; RRID: AB_2563905 |
| Brilliant Violet® 605 anti‐mouse CD11b | Biolegend | Cat# 117349; RRID: AB_2563905 |
| APC anti‐mouse CD103 | Biolegend | Cat# 12141; RRID: AB_1227503 |
| Brilliant Violet® 421 anti‐mouse CX3CR1 | Biolegend | Cat# 149023; RRID: AB_2565706 |
| Brilliant Violet® 421 anti‐mouse CD3e | Biolegend | Cat# 100341; RRID: AB_2562556 |
| PE anti‐mouse CD314 (NKG2D) | Biolegend | Cat# 130207; RRID: AB_1227713 |
| APC anti‐T‐bet | Biolegend | Cat# 644813; RRID: AB_10896913 |
| Alexa Fluor® 488 anti‐mouse FOXP3 | Biolegend | Cat# 126405; RRID: AB_1089114 |
| PE anti‐mouse ROR GAMMA (T) (B2D) | Thermo Fisher Scientific | Cat# 12‐6981‐80; RRID: AB_10805392 |
| PE‐Cy™7 anti‐mouse CD4 | BD biosciences | Cat# 552775; RRID: AB_394461 |
| anti‐CRAMP pAb | Proteintech | Cat# 12009‐1‐AP; RRID: AB_908736 |
| anti‐E‐cadherin pAb | BD biosciences | Cat# 610181; RRID: AB_397580 |
| anti‐F4/80 mAb | Abcam | Cat# ab6640; RRID: AB_1140040 |
| anti‐Ly6G mAb | Abcam | Cat# ab25377; RRID: AB_470492 |
| CD8a Antibody, Biotin | Miltenyi Biotec | Cat# 130‐118‐147; RRID: AB_2733536 |
| CD11b Antibody, Biotin | Miltenyi Biotec | Cat# 130‐113‐233; RRID: AB_2726044 |
| CD11c Antibody, Biotin | Miltenyi Biotec | Cat# 130‐113‐578; RRID: AB_2726178 |
| CD19 Antibody, Biotin | Miltenyi Biotec | Cat# 130‐113‐729; RRID: AB_2726270 |
| CD45R(B220) Antibody, Biotin | Miltenyi Biotec | Cat# 130‐123‐853; RRID: AB_2819526 |
| CD49b(DX5) Antibody, Biotin | Miltenyi Biotec | Cat# 130‐101‐934; RRID: AB_2660466 |
| CD105 Antibody, Biotin | Miltenyi Biotec | Cat# 130‐101‐992; RRID: AB_2660100 |
| MHC Class II Antibody, Biotin | Miltenyi Biotec | Cat# 130‐101‐849; RRID: AB_2660066 |
| Ter‐119 Antibody, Biotin | Miltenyi Biotec | Cat# 130‐120‐828; RRID: AB_2784480 |
| TCRγ/δ Antibody, Biotin | Miltenyi Biotec | Cat# 130‐114‐028; RRID: AB_2733574 |
| CD25 Antibody, Biotin | Miltenyi Biotec | Cat# 130‐092‐569; RRID: AB_871645 |
| Streptavidin MicroBeads | Miltenyi Biotec | Cat# 130‐048‐101 |
| CD45RB Antibody, FITC | Miltenyi Biotec | Cat# 130‐102‐461; RRID: AB_2658357 |
| Anti‐FITC MicroBeads antibody | Miltenyi Biotec | Cat# 130‐048‐701; RRID: AB_244371 |
| Oligonucleotides and other sequence‐based reagents | ||
| PCR primers | This study | Table EV2 |
| Chemicals, Enzymes and other reagents | ||
| CRAMP | GL biochem | Cat#088328 |
| Gliadin from wheat | Sigma | Cat#G3375‐25G |
| Gluten from wheat | Sigma | Cat#G5004‐500G |
| Software | ||
| FlowJo | BD Bioscience | RRID: SCR_008520; https://www.flowjo.com/solutions/flowjo/downloads |
| GraphPad Prism | GraphPad | RRID: SCR_002798; https://www.graphpad.com/ |
| Image J | Image J | RRID:SCR_003070; https://imagej.en.softonic.com/ |
| R project | R Project | RRID:SCR_001905; https://www.r‐project.org/ |
| FluorChem FC3 | ProteinSimple | RRID:SCR_013724; http://www.proteinsimple.com/fluorchem_e.html |
| ZEN | Zeiss | RRID:SCR_018163; http://stmichaelshospitalresearch.ca/wp‐content/uploads/2015/09/ZEN‐Black‐Quick‐Guide.pdf |
| Other | ||
| Illumina MiSeq | Illumina |
RRID:SCR_010233 |
| CRAMP ELISA kit | My biosource | Cat# MBS7700441 |
| LL‐37 ELISA kit | My biosource | Cat# MBS3800861 |
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