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. 2021 Jul 20;13(8):e13901. doi: 10.15252/emmm.202013901

Figure EV3. Modulation of glycolysis and other metabolic pathways during productive or latent HIV‐1 infection in microglia cells.

Figure EV3

Latently HIV‐1‐infected (HC69) or uninfected (C20) microglia cells were subjected to metabolomic analysis under unstimulated conditions or following stimulation with TNF to reactivate latent HIV‐1.
  • A, B
    Metabolite enrichment analysis in latently infected as compared to uninfected cells (A) or in cells reactivated from latency as compared to latently infected cells (B). The top enriched pathways were ordered according to P‐values obtained by Q statistics for metabolic data sets performed with Globaltest (MetaboAnalyst) (Xia et al, 2009).
  • C
    Heatmaps of glycolytic metabolites in latently infected HC69 cells as compared to their uninfected counterparts or HC69 cells with HIV‐1 reactivated by TNF as compared to latently infected cells. Data are displayed as Log2 fold change expression. Adjusted P‐values (q values) were calculated by the Benjamini–Hochberg false discovery rate.
  • D, E
    Relative ratios of NADH/NAD+ (D) and ATP/ADP (E) in latently infected and reactivated cells. Data were normalized using the matching uninfected control.