Figure 2.

Sulforaphane reduced cisplatin-induced hearing loss and hair cell damage in rats in vivo. A: The auditory brainstem response thresholds increased less, the shifts were statistically significant, including 4, 8, 16, 24, 32 kHz, and broad-band clicks, when the rats were injected with sulforaphane before cisplatin as compared to rats injected with cisplatin only (^***p < 0.001; n = 60 ears). B: The middle turn of the organ of Corti phalloidin staining indicated that outer hair cell loss was less in the sulforaphane +Cis group (red: phalloidin, blue: DAPI. Scale bar = 25 μm). C: The percentage of missing outer hair cells was significantly less in the sulforaphane +Cis group compared to the Cis group in the middle turn of the basilar membrane (n = 30 ears, **p < 0.01).