Table 2.
Cell counts for LC neurons retrogradely labelled from the spinal dorsal horn with AAV2retro.eGFP and Ctb.
| Injected material (number of animals) | Subset | eGFP only | Ctb only | eGFP+Ctb+ | %eGFP only | %Ctb only | %eGFP+Ctb+ |
|---|---|---|---|---|---|---|---|
| AAV2retro.CAG.eGFP + 0.5% Ctb (n = 4) | All LC neurons | 42 (4–18) | 275 (29–124) | 62 (11–21) | 13.0 (3.6–28.1) | 68.8 (45.3–88.6) | 18.2 (7.9–26.6) |
| TH+ | 1 (0–1) | 185 (20–90) | 8 (0–4) | 1.1 (0–4.3) | 94.3 (87–100) | 4.6 (0–8.7) | |
| TH– | 41 (4–17) | 90 (9–34) | 54 (7–19) | 21.9 (10.9–41.5) | 48.2 (22–73.9) | 29.9 (15.2–38.2) | |
| AAV2retro.hSyn.eGFP + 0.5% Ctb (n = 4) | All LC neurons | 40 (5–16) | 145 (24–49) | 65 (7–25) | 15.7 (10.4–18.4) | 58.7 (54.4–63.2) | 25.6 (18.4–33.3) |
| TH+ | 2 (0–1) | 133 (22–43) | 3 (0–3) | 1.5 (0–4.3) | 96.4 (91.3–100) | 2.2 (0–6.5) | |
| TH– | 38 (5–16) | 12 (1–6) | 62 (7–25) | 33.9 (22–40) | 10.7 (4.5–13.3) | 55.4 (46.7–72.7) |
Numbers indicate the total counted neurons per group, with the range of neurons counted per animal in parentheses. Percentages are an average value, with the range included in parentheses.