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. 2021 Aug 9;12:448. doi: 10.1186/s13287-021-02530-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Fusion index and hybrid myotube presence in either human bone marrow-derived mesenchymal stromal cells (hMSC) or pig bone marrow-derived mesenchymal stem cells (pMSC) co-culture with myoblasts: mouse primary myoblasts (mPM) or C2C12 myoblasts. A Fusion index of mPM and hMSC or pMSC co-cultured in MSCmed or PMmed under normoxic (NORM) or hypoxic (HYPO) conditions. B Fusion index of C2C12 myoblasts and hMSC or pMSC co-cultured in 3:2.5; 3:5; 3:7.5 ratio, in C2C12med, under normoxic (NORM) or hypoxic (HYPO) conditions. C Percentage of hybrid myotubes in mPM and hMSC or pMSC co-cultures in MSCmed or PMmed under normoxic (NORM) or hypoxic (HYPO) conditions. D Immunolocalization of hybrid myotubes in mPM and hMSC co-cultures, red—skeletal myosin, blue—cell nuclei, green human cell nuclei. E Percentage of hybrid myotubes in C2C12 and hMSC or pMSC co-cultures in C2C12med under normoxic (NORM) or hypoxic (HYPO) conditions. F Immunolocalization of hybrid myotubes in C2C12 and hMSC co-cultures, red—skeletal myosin, blue—cell nuclei, green—human cell nuclei. P value: *< 0.05; **< 0.01; ***< 0.001; ****< 0.0001