Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
Lactobacillus plantarum	K.R. Patil Laboratory‐Blasche S.	LPS
S90, Saccharomyces cerevisiae (MATα GAL2)	Steinmetz et al, 2002	S90
Lactococcus lactis	Blasche et al (2021)	17S
Brevibacterium casei	Blasche et al (2021)	303S
Δrib4rib5 (S90, rib4rib5:kanMX4:Hyg)	This study	DK1
Δrib4rib5RFP (S90, rib4rib5:kanMX4:Hyg, rfp:Clonat)	This study	DK2
Δabz1 (S90:Hyg)	This study	DK3
Recombinant DNA
puG6	Euroscarf	Cat #P30114
paG32	Addgene	Cat #35122
pCfB2049	Stovicek et al (2015)
Oligonucleotides
PCR primers	This study	Appendix Table S5
Software
Breseq	https://barricklab.org/twiki/pub/Lab/ToolsBacterialGenomeResequencing/documentation/index.html Deathrage and Barrick (2014)	N/A
PEAR	https://cme.h‐its.org/exelixis/web/software/pear/ Zhang et al (2014)	N/A
SPAdes (version 3.15.1)	https://cab.spbu.ru/software/spades/ Nurk et al (2013)	N/A
RAST	https://rast.nmpdr.org Overbeek et al (2014)	N/A
Netlogo	https://ccl.northwestern.edu/netlogo/ Wilensky (1999)	N/A
Matlab (version 2018a)	https://www.mathworks.com/products/matlab.html	N/A
R A Language for Data Analysis and Graphics (version 3.6.3)	https://www.r‐project.org	N/A
ImageJ	https://imagej.nih.gov/ij/ Schindelin et al (2012)	N/A
Konstantinidis_ALE_loop.nlogo	This paper	N/A
Other
1290 Infinity II LC system	Agilent	N/A
Exion LC system	Sciex	N/A
Shimadzu TQ8040 GC	Shimadzu	N/A
Synergy HTX	Biotech	N/A
ACQUITY UPLC/UHPLC System	Waters	N/A
GeneVac EZ‐2 plus evaporating system	SP Scientific	N/A
Riboflavin	Sigma‐Aldrich	Cat #R4500
FAD	Sigma‐Aldrich	Cat # F6625
FMN	Sigma‐Aldrich	Cat #F2253
Folate	Sigma‐Aldrich	Cat #F7876
Amino Acids	Sigma‐Aldrich	Appendix Table S6