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. 2021 Jul 23;11:715593. doi: 10.3389/fonc.2021.715593

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Copyright © 2021 Yang, Chen, Zhang, Xiong, Wang, Fang, Li, Fei and Gong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of ME2 promotes proliferation of GBM cells. (A) ME2 overexpression detected by real-time PCR in SW1783 and U251MG cells (**P < 0.01). (B) Western blotting to confirm ME2 expression in in SW1783 and U251MG cells transfected with vector or Flag-ME2 plasmids. (C, D) Effects of ME2 overexpression on proliferation of SW1783 and U251MG cells determined by CCK-8 assay (*P < 0.05). (E, F) Colony formation assay to determine the colony formation of glioma cells (***P < 0.0001).