FIGURE 4:

Cortical rotation is dependent on the formin CYK-1. (A) Phenotype frequency for various genetic perturbations of unc-61 and cyk-1. Alternating rotation changes direction at least once; continuous rotation has a single period of increased velocity; intermittent rotation has several periods interspersed with periods of slower or no movement; none indicates that no rotation occurred. (B) Example kymographs of cells exhibiting reversed handedness or alternating rotation. (C, D) Circumferential velocities over time averaged across the anterior–posterior axis. Colored lines indicate individuals of the corresponding color-coded phenotype; purple: control average; red: cyk-1(RNAi) population average (n = 10); brown: cyk-1(RNAi); unc-61(e228) population average (n = 18); dashed line: reversed handedness. (E–H) Quantification of mean velocity, maximum velocity, duration, and displacement, respectively. Colored dots are individuals of the corresponding phenotype. Vertical notches are population means; horizontal lines are 95% confidence intervals. *p < 0.05; unmarked pairings are not significantly different. (I) Ratio of LifeAct fluorescence in the zygote anterior vs. posterior in anaphase in control (gray) and CYK-1–depleted cells (black; open: continuous and intermittent rotation with right-handed chirality; filled: rotation with continuous left-handed chirality [n = 3] or alternating handedness [n = 2]) **p < 0.01; ****p < 0.001.