FIGURE 2:

Peroxisomal membranes undergo rearrangement in SARS-CoV-2-infected Huh-7 cells. Cells were grown on coverslips, infected with SARS-CoV-2 at a MOI of 1 or mock-infected, and fixed at 24, 48, and 72 hpi. Cells were labeled with antibodies against PMP70 and PEX14. Cell nuclei were stained with DAPI. Cells were observed by IF confocal microscopy. Images are maximum intensity projections. Black and white images show single labeling by PMP70 or PEX14 antibodies. Squares in a, e, I, and m are at higher magnification in d, h, l, and p, respectively. Bars: 10 μm (a–c, e–g, i–k, m–o), 2 μm (d, h, l, p). Also see Supplemental Video 1.