FIGURE 6:

FAM134C expression elevates upon starvation. (A) Western blots and quantifications showing the changes in FAM134C levels at normal growth conditions (DMEM) and upon amino acid starvation (EBSS) with/without bafilomycin A1 in U2-OS cells at both high and low cell confluency, silencing of FAM134C in Huh-7 cells (B), and upon DTT-induced ER stress in Huh-7 cells (C). LC3II was used as a general reporter for autophagy and Phospho-elF2α as a marker for ER stress. GAPDH and α-tubulin were used as loading controls. For quantification, the relative average signal was obtained by normalizing for the signal from cells after a 1 h incubation in DMEM. Error bars indicate ±SEM.