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. 2021 Jun 1;32(12):1158–1170. doi: 10.1091/mbc.E20-06-0409

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© 2021 Kumar, Lak, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 7: — FAM134C is required for amino acid starvation–induced ER-phagy. Confocal LM images of U2-OS cells expressing RAMP-eGFP-mCherry and mock, FAM134C shRNAs 1–3, or RTN4 siRNA at basal or amino acid starvation (EBSS) conditions. Red puncta represent acidified ER. Insets show higher magnification of boxed areas. The percentage of cells having acidified ER was calculated from three to 10 experimental replicates. Error bars indicate ±SEM. Total number of cells analyzed per condition was 176, 88, 26, 19, 119, 202, 74, 34, 24, and 120 according to the order in the graph. Scale bars: 10 µm.