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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Chemosphere. 2021 Mar 24;277:130355. doi: 10.1016/j.chemosphere.2021.130355

Figure 4.

Figure 4.

(A) Representative recordings of Ca2+ sparks in a quiescent mouse ventricular myocyte under BPA-free control and 6 min after exposure to solution prepared using water extract from unwashed tested bottles (No wash). (B) Average Ca2+ spark frequency (left) and peak spark amplitude (right) under control and upon exposure to indicated solutions. N = 15, 8, and 7 myocytes for control, no wash, and dishwashing 6x group, respectively. (C) Representative images of L. variegatus showing the DBV and pulses propagating along the vessel (arrow), after 7-day exposure to control BPA-free solution and solution prepared using water extract from no wash bottles. (D) Average DBV pulsing rate in control and treated groups. N = 21 and 22. ** P < 0.001, # P > 0.1 vs control in unpaired t-test. Error bars are S.D.