FIG 4.

Determination of the essential genes of WT GBS and PBP deletion mutant strains. (A to E) Dot plots showing the insertion index of each gene in the GBS genome in the WT background and in each PBP deletion background. Insertion index scores of cell wall biosynthesis genes pbp1a, pbp1b, pbp2a, pbp2b, and rodA are highlighted in color. Pseudogene 1280 is highlighted in black as a control, with the assumption that insertion inactivation of a pseudogene should not affect bacterial fitness. The dotted lines denote the essential change point predicted by TraDIS. Data points below the dotted lines are predicted essential genes. (F) Venn diagram portraying the predicted essential genomes of WT GBS and PBP deletion mutant strains.