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Transposon insertion profile showing the impact of deleting pbp2b or pbp1a on the essentiality of other peptidoglycan synthesis genes. (A) Transposon insertion into pbp1a is not tolerated in the pbp2b deletion background, except for the 3′ terminal last ∼10% of the gene. (B and C) Transposon inactivation of pbp2b or rodA is not tolerated in the pbp1a deletion background. The locus tag numbers refer to the annotation for GBS strain CNCTC10/84.
