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Supercoiling status of reporter plasmid pACYC184 in topA+ and topA derivatives, as determined by chloroquine-agarose gel electrophoresis. Genotypes at topA and rpoB loci are indicated on top of each lane; for topA, Δ and 480 refer to ΔtopA::FRT and topA-Ins480::FRT, respectively. Strains were pACYC184 derivatives: lane 1, GJ12134; lane 2, GJ16819; lane 3, 18976; lane 4, GJ18977; lane 5, GJ17776; lane 6, GJ17777; lane 8, GJ18601; lane 9, GJ18910. Lane 7, DNA size standards.
