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Synthetic topA rnhA lethality, suppressed by ΔdnaA. Blue-white screening assay at 30°C on glucose minimal medium A with the topA+ dnaA+ shelter plasmid pHYD2390 in MG1655 Δtus rpoB*35 derivatives carrying different alleles of topA, rnhA, and dnaA as indicated at the top of each panel. Strains employed for the different panels were pHYD2390 derivatives: i, GJ17784; ii, GJ19609; iii, GJ18951; iv, GJ17783; v, GJ19608; and vi, GJ18983.
