FIG 1.

GraS EL is essential for sensing and survival with CAMPs. (a) mprF promoter fusion assays using pSK236::P_mprf-mRuby. Overnight cultures were diluted to an OD₆₀₀ of 1.6 and incubated for 50 min at 37°C with shaking. Fluorescence was read with an excitation wavelength of 558 nm and an emission wavelength of 592 nm. (b) Cytochrome c binding assays. Overnight cell cultures with an OD₆₅₀ of 3 were incubated for 10 min with 0.5 mg/ml cytochrome c to assess differences in binding by measuring supernatant A₅₃₀. (c) Two-hour time-kill assays. Cells were diluted to 10⁶ CFU/ml and incubated with 32 μg/ml PMB at 37°C for 2 h with shaking at 250 rpm. The graph shows the percent difference between colony counts plated at 0 h and 2 h, standardized to 100% colony recovery from strain NIH051475. Each experiment represents means and standard deviations of three biological replicates. Statistical analysis was conducted by multiple-comparison one-way analysis of variance (ANOVA) against strain NIH051475 using the Dunnett post hoc multiple-comparison correction. **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; n.s., not significant.