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Inhibition of carD promoter transcription by CarD is reduced by substitutions in CarD. (A) In vitro transcription of the carD promoter with 20 nM RNAP without CarD or with wild-type or W91A, W91L, or Q31A/I33A/R53A triple-mutant CarD (1,280 nM). (B) Transcription from the carD promoter in the presence of wild-type or mutant CarD relative to its transcription in the absence of CarD from experiments like that shown in panel A; error bars indicate range from 2 assays.
