TABLE 2:
PIP3 generation properties of Gγ mutants.
| Gγ type | Sequence | Rate of PIP3 generation (10–3 s–1) | Extent of PIP3 generation (AFU) |
|---|---|---|---|
| Gγ3 WT | NPFREKKFFCALL | 6.7 ± 0.3 | 76.6 ± 7.4 |
| Gγ3-FF→GG | NPFREKKGGCALL | 4.0 ± 0.1 | 47.1 ± 7.3 |
| Gγ3-FF shifted | NPFFFREKKCALL | 4.4 ± 0.6 | 56.6 ± 8.5 |
| Gγ3-KK→GG in FF shifted | NPFFFREGGCALL | 4.2 ± 0.6 | 68.4 ± 6.4 |
| Gγ3-F65→G | NPGREKKFFCALL | 4.0 ± 0.4 | 54.9 ± 6.2 |
| Gγ3-RE→GGGG | NPFGGGGKKFFCALL | 5.1 ± 0.2 | 51.3 ± 7.1 |
| Gγ9 WT | NPFKEKGGCLIS | 2.1 ± 0.3 | 18.3 ± 2.5 |
| Gγ9-GG→FF | NPFKEKFFCLIS | 4.5 ± 0.5 | 46.2 ± 4.9 |
| Gγ9-KEK→GGG | NPFGGGGGCLIS | 2.7 ± 0.4 | 33.9 ± 5.5 |
| Gγ9 with Gγ3 pre-CaaX | NPFREKKFFCLIS | 4.0 ± 0.7 | 49.1 ± 3.8 |