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. 2021 Aug 1;32(16):1501–1513. doi: 10.1091/mbc.E21-05-0258

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© 2021 van Loon et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 6: — Short-term inhibition of NMII contractility alters microridge patterns in individual cells. (A) Representative visualizations of microridge distances, orientations, and alignment domains in periderm cells expressing Lifeact-GFP before (48 hpf, 0 h) and after (49 hpf, 1 h) 1-h treatment with 50 μM blebbistatin or vehicle (DMSO). (B) Line plot of microridge density, defined as the sum microridge length (μm) normalized to apical cell area (μm²), from periderm cells before (48 hpf, 0 h) and after (49 hpf, 1 h) 1-h treatment with 50 μM blebbistatin or vehicle control (DMSO). DMSO, n = 22 cells from 4 fish; 50 μM blebbistatin, n = 25 cells from 4 fish. P = 4.09 × 10^–11, one-way repeated measures ANOVA. (C) Line plot of microridge spacing mode from periderm cells before (48 hpf, 0 h) and after (49 hpf, 1 h) 1-h treatment with 50 μM blebbistatin or vehicle control (DMSO). DMSO, n = 22 cells from 4 fish; 50 μM blebbistatin, n = 25 cells from 4 fish. P = 7.76 × 10^–6, one-way repeated measures ANOVA. (D) Line plot of microridge spacing variability (interquartile range of distances) between neighboring microridges in periderm cells before (48 hpf, 0 h) and after (49 hpf, 1 h) 1-h treatment with 50 μM blebbistatin or vehicle control (DMSO). DMSO, n = 22 cells from 4 fish; 50 μM blebbistatin, n = 25 cells from 4 fish. P < 2 × 10^–16, one-way repeated measures ANOVA. (E) Line plot of the alignment index in periderm cells before (48 hpf, 0 h) and after (49 hpf, 1 h) 1-h treatment with 50 μM blebbistatin or vehicle control (DMSO). Note that control treatment with DMSO decreased alignment, likely reflecting disruption of the pattern by the mounting and unmounting procedure required for this experiment, but treatment with blebbistatin increased alignment, emphasizing the role of NMII in this process. DMSO, n = 22 cells from 4 fish; 50 μM blebbistatin, n = 25 cells from 4 fish. P = 1.02 × 10^–8, one-way repeated measures ANOVA. Scale bars: 5 μm (A). ***p ≤ 0.001.